Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC) (CAT#: HPAB-AP684-YC)
This product is a recombinant mouse antibody that recognizes IL17RB. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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DB
Figure 1 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC) in DB
Dot Blot analysis of HPAB-AP684-YC was performed by coating with human IL17RB protein (His tag).
HPAB-AP684-YC incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
WB
Figure 2 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC) in WB
Western blot analysis of HPAB-AP684-YC was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP684-YC and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 3 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC) in ELISA
ELISA analysis of HPAB-AP684-YC was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with HPAB-AP684-YC. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human IL17RB
- Species Reactivity
- Human, Mouse
- Applications
- Neut, FuncS, IHC, ELISA, FC, Inhib
- Related Disease
- Asthma, Ulcerative colitis and Crohn's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1426-VHH | Recombinant Anti-Human IL17RB VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-AP684-YC, RRID: AB_3111432)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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